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Metadata: Microplastic effect data. The world is your oyster: low-dose, long-term microplastic exposure of juvenile oysters
Abstract:
This dataset contains three separate files form an eighty day exposure study where we exposed juvenile oysters, Crassotrea gigas, to 3 different MP concentrations (10Power4, 10Power5, 10Power6 particles LPower minus 1), represented by 6 Microns Polysyrene (PS) microbeads, compared to a control treatment receiving no MP. There is a data file for shell growth and length, one for condition index and one for lysosomal stability.
Data holder:
Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)
| Other details | ||
| Internal code | Internally assigned metadata identifier | 9135 |
| Title | The title is used to provide a brief and precise description of the dataset such as 'Date', 'Originating organisation/programme', 'Location' and 'Type of survey'. All acronyms and abbreviations should be reproduced in full. | Microplastic effect data. The world is your oyster: low-dose, long-term microplastic exposure of juvenile oysters |
| File Identifier | The File Identifier is a code, preferably a GUID, that is globally unique and remains with the same metadata record even if the record is edited or transferred between portals or tools. | CEFAS11ebd3ad-c4fb-4b58-85de-118da2fbed08 |
| Resource Identifier | This is the code assigned by the data owner. | CEFAS20201 |
| Resource type | The resource type will likely be a dataset but could also be a series (collection of datasets with a common specification) or a service. | dataset |
| Start date | This describes the date the resource starts. This may only be the year if month and day are not known | 2012-06-30 |
| End date | This describes the date the resource ends. This may only be the year if month and day are not known | 2019-12-13 |
| Frequency of updates | This describes the frequency with which the resource is modified or updated i.e. a monitoring programme that samples once per year has a frequency that is described as 'annually'. | notPlanned |
| Abstract | The abstract provides a clear and brief statement of the content of the resource. | This dataset contains three separate files form an eighty day exposure study where we exposed juvenile oysters, Crassotrea gigas, to 3 different MP concentrations (10Power4, 10Power5, 10Power6 particles LPower minus 1), represented by 6 Microns Polysyrene (PS) microbeads, compared to a control treatment receiving no MP. There is a data file for shell growth and length, one for condition index and one for lysosomal stability. |
| Lineage | Lineage includes the background information, history of the sources of data, data quality statements and methods. | Shell Length & Weight: Shell height, the maximum dimension from hinge to growth edge, is commonly referred to as shell length, which will be used to describe this dimension here. The shell length of every oyster was measured to the nearest mm. Additional dimensions were measured to account for irregular oyster shapes (e.g. long and thin). All measurements (±1.0 mm) were taken using a digital calliper system that enabled the rapid recording of data. In the weighing technique, oysters were air dried at room temperature for 5 minutes and weighed to the nearest 0.0001g. Oyster meat was oven dried to constant weight (68C for 48 hours) and then meat and shell were weighed separately to the nearest 0.0001g, after a short cooling period. Condition Index; The CI of bivalves is measured by relating either the weight or volume of the meat to some aspect of the shell. In the current study, oyster shell length and weight measurements were standardized using the following formula: Condition Index = (dry meat weight in g) * 100 / (shell weight in g). This widely-used condition index, because of the nature of the measurements involved, is easily standardized and is thus used globally. In addition, the use of dry tissue weights eliminates the bias due to water content fluctuations of whole tissue. A low value for this index indicates that a major biological effort has been expended, either as maintenance energy under poor environmental conditions or disease, or in the production and release of gametes. Thus, as an indicator of stress, or sexual activity, this index gives meaningful information about the physiological state of the animal. Lysosomal Membrane Stability; A series of solutions and reagents were used to test LMS. A lysosomal membrane labilising buffer (Solution A) was made with 0.1M Na-citrate Buffer - 2.5% NaCl w:v (pH 4.5). The substrate incubation medium (Solution B) consisted of 20 mg of N-Acetyl-ß-hexosaminidase (Sigma, N4006) or Napthol AS-BI phosphate (Sigma N2125), dissolved in 2.5 mL of 2-methoxyethanol (Merck, 859) and made up to 50 mL with solution A. This solution contained 3.5 g of collagen-derived polypeptide (POLYPEP, P5115 Sigma) as low viscosity polypeptide to act as a section stabiliser. This solution was prepared 5 minutes before use. The diazoniumdye (Solution C) contained 0.1M Na-phosphate buffer (pH 7.4) containing 1 mg mL-1 of diazonium dye Fast Violet B salts (Sigma, F1631). The fixative (Solution D) was made from Baker’s calcium formol containing 2.5% NaCl (w:v). An aqueous mounting medium (Vector Laboratories H1000, Kaiser glycerine gelatine, Difco, Sigma) was used. The lysosomal membrane stability was cytochemically determined using N-Acetyl-ß-hexosaminidase. Cryostat sections were cut at 8-10µm (in duplicate on the same slide) and left in the cryostat chamber until just before use. Seven slides were prepared in this manner. Solution A was placed into a water bath at 37 °C to acclimatise. The slides were placed into pre-treatment solution A so that each slide had a different pre-treatment time of 30, 25, 20, 15, 10, 5, and 2 minutes i.e. slide 7= 30 minutes, slide 6 = 25 minutes, slide 5= 20 minutes, etc. Following pre-treatment, slides were transferred to solution B for 20 minutes at 37 °C in a staining jar in a shaking water-bath. The slides were rinsed with a saline solution (3.0% NaCl) at 37 °C for 2 to 3 minutes. The slides were then transferred to solution C at room temperature for 10 minutes. Following this, slides were rinsed rapidly in running tap water for 5 minutes. Sections were fixed for 10 minutes in Solution D pre-cooled to 4 °C. Finally, slides were rinsed in distilled water, mounted in aqueous mounting medium and analysed. The labilisation period (LP) is the time of pre-treatment required to labilise the lysosomal membranes fully, resulting in maximal staining intensity for the enzyme being assayed. The staining intensity was assessed visually using microscopic examination. The labilisation period can be effectively measured by microscopic assessment of the maximum staining intensity in the pre-treatment series, a microdensitometer is not completely necessary for accurate determination. All assessments were carried out on duplicate sections for each digestive gland at each pre-treatment time. Lysosomes will stain reddish-purple due to the reactivity of the substrate with N-acetyl-ß-hexosaminidase. The LP for each section corresponds to the average incubation time in the acid buffer that produces maximal staining reactivity. LP for the other replicate is similarly obtained. Finally, a mean value of LMS of the sample was calculated utilizing the data obtained from the 10 animals analysed. Determination of the LP is usually quite straightforward, but a complicating situation occasionally arises in which the pre-treatment series shows two peaks of staining intensity, possibly due to differential latent properties of the subpopulations of lysosomes. In this situation, the first peak of activity was used to determine labilisation period as it is the most responsive to staining. |
| Related keywords | ||
| Keyword | General subject area(s) associated with the resource, uses multiple controlled vocabularies | Biomarker |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Ecotoxicity | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Bioassay and contaminant biological impact | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Species distribution | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Water column | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Marine Environmental Data and Information Network | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | data.gov.uk | |
| Geographical coverage | ||
| North | The northern-most limit of the data resource in decimal degrees | 52.4595 |
| East | The eastern-most limit of the data resource in decimal degrees | 1.74086 |
| South | The southern-most limit of the data resource in decimal degrees | 52.4581 |
| West | The western-most limit of the data resource in decimal degrees | 1.73881 |
| Responsible organisations | ||
| Role | The point of contact is person or organisation with responsibility for the creation and maintenance of the metadata for the resource. | pointOfContact |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The originator is the person or organisation who created, collected or produced the resource. | originator |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The custodian is the person or organisation that accepts responsibility for the resource and ensures appropriate care and maintenance. If a dataset has been lodged with a Data Archive Centre for maintenance then this organisation is be entered here. | custodian |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The distributor is the person or organisation that distributes the resource. | distributor |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The owner is the person or organisation that owns the resource. | owner |
| Organisation name | European Commission | |
| info@publications.europa.eu | ||
| Resource locators | ||
| Locator URL | Web address (URL) that links to the resource | https://data.cefas.co.uk/view/20201 |
| Locator name | Name of the web resource | Cefas Data Portal |
| Dataset constraints | ||
| 20.1 Limitations on Public Access - Access constraints | This states `otherRestrictions` from ISO vocabulary RestrictionCode and is an INSPIRE/GEMINI requirement. | otherRestrictions |
| 20.2 Limitations on Public Access - Other constraints | noLimitations | |
| 21.1 Conditions for Access and Use - Use constraints | This states `otherRestrictions` from ISO vocabulary RestrictionCode and is an INSPIRE/GEMINI requirement. | otherRestrictions |
| 21.2 Conditions for Access and Use - Other constraints | http://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_RestrictionCode | |
| Version info | ||
| Date of publication | The publication date of the resource or if previously unpublished the date that the resource was made publicly available via the MEDIN network. | 2019-12-18 |
| Date of last revision | The most recent date that the resource was revised. | 2024-07-24 |
| Date of creation | The date that the resource was created. | 2019-12-12 |
| Harvest date | The date which this record has been (re)harvested from the provider. | 2026-04-12 |
| Metadata date | The date when the content of this metadata record was last updated. | 2024-07-24 |
| Metadata standard name | The name of the metadata standard used to create this metadata | MEDIN |
| Metadata standard version | The version of the MEDIN Discovery Metadata Standard used to create the metadata record | 3.1.1 |