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Metadata: Northwest European shelf seas - Phytoplankton pigments - 2020 to 2023
Abstract:
These data contain concentrations in micrograms per litre (ug/L) of the individual accessory pigments of phytoplankton measured by High Performance Liquid Chromatography (HPLC). Pigment samples were taken on Cefas fisheries surveys in the Celtic Seas, and sent to DHI laboratory in Denmark for HPLC analysis. Data was then sent back to Cefas and matched with metadata and quality controlled. Chlorophyll concentrations from this dataset have a variety of uses: for assessment of concentrations for purposes such as eutrophication assessments, validation of remote sensing derived chlorophyll estimates, as a proxy for phytoplankton biomass, or to calibrate fluorometers. Pigment concentrations can be applied like a fingerprint to derive phytoplankton functional types and give information about the phytoplankton community.
Data holder:
Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)
| Other details | ||
| Internal code | Internally assigned metadata identifier | 9923 |
| Title | The title is used to provide a brief and precise description of the dataset such as 'Date', 'Originating organisation/programme', 'Location' and 'Type of survey'. All acronyms and abbreviations should be reproduced in full. | Northwest European shelf seas - Phytoplankton pigments - 2020 to 2023 |
| File Identifier | The File Identifier is a code, preferably a GUID, that is globally unique and remains with the same metadata record even if the record is edited or transferred between portals or tools. | CEFAS6e781511-47c3-4629-aa5e-6a9d6e102f5f |
| Resource Identifier | This is the code assigned by the data owner. | CEFAS21386 |
| Resource type | The resource type will likely be a dataset but could also be a series (collection of datasets with a common specification) or a service. | dataset |
| Start date | This describes the date the resource starts. This may only be the year if month and day are not known | 2020-10-05 |
| End date | This describes the date the resource ends. This may only be the year if month and day are not known | 2023-10-26 |
| Frequency of updates | This describes the frequency with which the resource is modified or updated i.e. a monitoring programme that samples once per year has a frequency that is described as 'annually'. | annually |
| Abstract | The abstract provides a clear and brief statement of the content of the resource. | These data contain concentrations in micrograms per litre (ug/L) of the individual accessory pigments of phytoplankton measured by High Performance Liquid Chromatography (HPLC). Pigment samples were taken on Cefas fisheries surveys in the Celtic Seas, and sent to DHI laboratory in Denmark for HPLC analysis. Data was then sent back to Cefas and matched with metadata and quality controlled. Chlorophyll concentrations from this dataset have a variety of uses: for assessment of concentrations for purposes such as eutrophication assessments, validation of remote sensing derived chlorophyll estimates, as a proxy for phytoplankton biomass, or to calibrate fluorometers. Pigment concentrations can be applied like a fingerprint to derive phytoplankton functional types and give information about the phytoplankton community. |
| Lineage | Lineage includes the background information, history of the sources of data, data quality statements and methods. | 2 litre water samples were collected on board the RV Cefas Endeavour from the continuous flow ferrybox water supply (4m depth), or from Niskin bottles on a rosette fired at particular depths. Water samples were filtered through a Whatman GFF filter, and immediately frozen in a -80°C freezer on board. Pigments were stored at -80°C for 1-4 months, before being selected for analysis, then transferred to the analysis laboratory (DHI; Horsholm, Denmark) using dry ice. Pigments were extracted adding 6 ml of 95% acetone (and an internal standard, vitamin E) to each filter. The samples were sonicated in an ice-cold sonication bath for 10 min and extracted at 4 °C for 20 hours. The samples were then filtered through 0.2 µm Teflon syringe filter into HPLC vials. Buffer and samples were injected on HPLC (Shimadzu LC-10A HPLC system with LC Solution software) in the ratio 5:2 using a pre-treatment program and mixing in the loop before injection (Schlüter et al 2016). The HPLC method used was the Van Heukelem & Thomas (2005) method where the gradient was adjusted to optimise the resolution of the pigments. The raw data were stored in PostgreSQL database. Quality control was performed following methods in Aiken et al 2009. Samples were removed if they had insufficient chlorophyll (<0.04ug/L), a ratio of above 0.5 of degradation pigments (chlorophyllide + pheophorbide + pheophytin) to chlorophyll a, or if there was a difference between total chlorophyll a (TChla) and accessory pigments (AP) of more than 40% of the total pigments. Additionally, where at least 5 samples were collected for a survey, total chlorophyll a was regressed against total accessory pigments, and the data from the survey was rejected if the regression was poor (r2<0.75, slope <0.65 or <1.4). This dataset is an update to Collingridge, Forster, Capuzzo and Creach. (2019). Northwest European shelf seas - Phytoplankton pigments - 2010 to 2019. Cefas, UK. V2. doi: `https://doi.org/10.14466/CefasDataHub.75`_ Aiken, J. et al. `Phytoplankton pigments and functional types in the Atlantic Ocean: A decadal assessment, 1995–2005`_. Deep Sea Res. Part II Top. Stud. Oceanogr.56, 899–917 (2009). van Heukelem, L. Van & Thomas, C. S. `The Second SeaWIFS HPLC Analysis Round-Robin Experiment`_ (eds. Hooker, S. B. et al.) 86–92 (NASA, 2005). Schlüter, L et al. `Comparing microscopic counts and pigment analyses in 46 phytoplankton communities from lakes of different trophic state`_. Freshwater Biology 61, 1627-1639 .. _`https://doi.org/10.14466/cefasdatahub.75`: https://doi.org/10.14466/CefasDataHub.75 .. _`phytoplankton pigments and functional types in the atlantic ocean: a decadal assessment, 1995–2005`: https://www.sciencedirect.com/science/article/abs/pii/S0967064508003184 .. _`the second seawifs hplc analysis round-robin experiment`: https://ntrs.nasa.gov/api/citations/20060003771/downloads/20060003771.pdf .. _`comparing microscopic counts and pigment analyses in 46 phytoplankton communities from lakes of different trophic state`: https://onlinelibrary.wiley.com/doi/10.1111/fwb.12803?msockid=31044fd6505f6ad018fa5a7151fb6b1c |
| Related keywords | ||
| Keyword | General subject area(s) associated with the resource, uses multiple controlled vocabularies | Chlorophyll pigment concentrations in the water column |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Phytoplankton | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Pigments | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Species distribution | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Epipelagic | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Marine Environmental Data and Information Network | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | data.gov.uk | |
| Geographical coverage | ||
| North | The northern-most limit of the data resource in decimal degrees | 62.5 |
| East | The eastern-most limit of the data resource in decimal degrees | 14 |
| South | The southern-most limit of the data resource in decimal degrees | 47.5 |
| West | The western-most limit of the data resource in decimal degrees | -13 |
| Responsible organisations | ||
| Role | The point of contact is person or organisation with responsibility for the creation and maintenance of the metadata for the resource. | pointOfContact |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The originator is the person or organisation who created, collected or produced the resource. | originator |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The custodian is the person or organisation that accepts responsibility for the resource and ensures appropriate care and maintenance. If a dataset has been lodged with a Data Archive Centre for maintenance then this organisation is be entered here. | custodian |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The distributor is the person or organisation that distributes the resource. | distributor |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The owner is the person or organisation that owns the resource. | owner |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| data.manager@cefas.co.uk | ||
| Resource locators | ||
| Locator URL | Web address (URL) that links to the resource | https://data.cefas.co.uk/view/21386 |
| Locator name | Name of the web resource | Cefas Data Portal |
| Dataset constraints | ||
| 20.1 Limitations on Public Access - Access constraints | This states `otherRestrictions` from ISO vocabulary RestrictionCode and is an INSPIRE/GEMINI requirement. | otherRestrictions |
| 20.2 Limitations on Public Access - Other constraints | noLimitations | |
| 21.1 Conditions for Access and Use - Use constraints | This states `otherRestrictions` from ISO vocabulary RestrictionCode and is an INSPIRE/GEMINI requirement. | otherRestrictions |
| 21.2 Conditions for Access and Use - Other constraints | http://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_RestrictionCode | |
| Version info | ||
| Date of publication | The publication date of the resource or if previously unpublished the date that the resource was made publicly available via the MEDIN network. | 2024-03-20 |
| Date of last revision | The most recent date that the resource was revised. | 2025-09-25 |
| Date of creation | The date that the resource was created. | 2021-11-25 |
| Harvest date | The date which this record has been (re)harvested from the provider. | 2026-04-19 |
| Metadata date | The date when the content of this metadata record was last updated. | 2025-09-25 |
| Metadata standard name | The name of the metadata standard used to create this metadata | MEDIN |
| Metadata standard version | The version of the MEDIN Discovery Metadata Standard used to create the metadata record | 3.1.1 |