CEFAS0361a083-bc34-428e-b9d0-41c422c2faad English dataset Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk pointOfContact 2024-07-12T10:20:20 EPSG Geodetic Parameter Registry 2022-01-07 revision WGS 84 North Sea phytoplankton pigments 2010 to 2011 2017-03-17 publication 2024-07-12 revision 2017-03-16 creation CEFAS18473 https://data.cefas.co.uk These data contain information about the individual accessory pigments of phytoplankton measured by High Pressure Liquid Chromatography (HPLC) from two North Sea International Bottom Trawl Surveys in 2010 and 2011. These have been applied like a 'fingerprint' to identify phytoplankton functional types (PFT's). Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk originator Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk custodian Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk distributor Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) data.manager@cefas.co.uk owner Department for Environment, Food and Rural Affairs (DEFRA) defra.helpline@defra.gov.uk owner European Commission info@publications.europa.eu owner quarterly Phytoplankton Pigments SeaDataNet P03 parameter discovery vocabulary 2011-03-25 revision Plankton Algal bloom GEMET, version 1.0 2008-06-01 publication Chlorophyll pigment concentrations in the water column SeaDataNet P02 parameter discovery vocabulary 2011-03-25 revision Habitats and biotopes GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Upper epipelagic Water column SeaVoX Vertical Co-ordinate Coverages 2010-05-18 revision Marine Environmental Data and Information Network MEDIN metadata record availability 2012-01-11 publication data.gov.uk MEDIN metadata record availability 2012-01-11 publication otherRestrictions noLimitations otherRestrictions Public data - no limitations to reuse English environment -13.00 14.00 47.50 62.50 2010-08-07 2011-09-04 Delimited Geographic Information System Unknown https://data.cefas.co.uk/view/18473 Cefas Data Portal download The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: CSV, ESRI Shapefile. The data can also be accessed via the WFS and WMS protocols. dataset INSPIRE Data Specification on Habitats and Biotopes – Technical Guidelines 2013-12-10 publication false See the referenced specification Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 publication true See the referenced specification 10L water samples were taken from niskin bottles on a rosette fired at various depths, or from the continuous flow ferrybox water supply (approx. 4m water depth) in adverse weather conditions. Water samples (typically 1000ml) were prefiltered with 200 micron gauze to remove larger zooplankton and debris, then filtered through a Whatman GFF filter, and immediately frozen in a -80 degrees C freezer on board. On return to shore, samples were transferred to a -80 degrees C freezer for a storage period of 1-2 months before shipping of samples on dry ice to an accredited HPLC laboratory (DHIWater Quality Institute; Horsholm,Denmark) for chlorophyll a (Chl a) quantification and full accessory pigment analysis. Pigments were extracted adding 6 ml of 95 percent acetone (and an internal standard, vitamin E) to each filter, at 4 degrees C for 20 hours. Samples were then filtered through 0.2 micron Teflon syringe filter into HPLC vials, together with DHI mixed pigments. Buffer and samples were injected on HPLC (Shimadzu LC-10A HPLC system with LC Solution software) in the ratio 5:2 using a pre-treatment program and mixing in the loop before injection. The HPLC method used was the HPL method by Van Heukelem and Thomas (2005). Pigment data from the surface stations were quality data controlled in several steps: first, with an initial comparison of HPLC Chl a against independent measures of chlorophyll fluorescence from the fluorometers on the ship’s FerryBox and CTD system. This step corrected a small number of mislabelled samples. In a second step, anomalies within a sample were detected using methods described by Aiken et al. (2009), e.g. regression of total accessory pigments against Chl a concentration and search for outliers. Diagnostic pigment analysis was then used on the quality controlled data set to relate the composition of specific accessory pigments to the relative contribution of different size classes to the total phytoplankton biomass. The designation of specific accessory pigments to algal taxonomic groups of different size, e.g. fucoxanthin and peridinin for large-cell diatoms and dinoflagellates, has been widely established in the biological oceanographic literature (Uitz et al., 2006, 2008). The equations used to estimate the contribution of pico-phytoplankton (0–2 microns), nano-phytoplankton (2–20 microns) and micro- or net phytoplankton (greater than 20 microns) were later modified by Hirata et al. (2008, 2011) and Brewin et al. (2010). The various methods differ in the degree to which the marker pigments chlorophyll b (Chl b) and 19-hexfucoxanthin (19-hex) are attributed to the three size classes. Here, Chl b and 19-hex were assigned equally to the picophytoplankton and nano-phytoplankton size classes. Pico-phytoplankton are therefore represented by zeaxanthin, Chl b, and 19-hex; nano-phytoplankton are represented by 19-hex, 19-but, alloxanthin, and Chl b; and micro-phytoplankton are represented by fucoxanthin and peridinin. Results are expressed as a proportion of the total Chl a concentration for each station. MEDIN 3.1.1