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biota samples (anchovies, redeye round herring and sardines) collected and 
analysed in South Africa in 2019. The file contains data of the samples 
weight, length and number of microplastic items found. Fish is ordered by 
'stratum' (geographical reference, please see 
South_Africa_Biota_Microplastics_Strata.doc). Fish of the same species in the 
same stratum were also stored in bags and a blank was measured in the lab to 
assess contamination while working with specimens from the same bag. Blank 
values are also reported. in the file. The file also reports information for 
each sample. Since fish populations were homogeneous in size and shape, body 
weight and length were recorded for only 5 individuals per bag, and averaged 
values are reported for all fish from that bag. Stomach weight was measured 
individually for all specimens. For sardines in strata E and F the lengths and 
weights of each fish were measured, allowing for further analysis on the 
health effects of microplastics in sardines. The file 
South_Africa_Biota_Microplastics_FishMeasurements.csv reports body weights and 
length of the specimen actually measured in each bag. A third file 
(South_Africa_Biota_Microplastics_FTIR.csv) contains the profiles of the 
ATR-FT-IR spectrum analysis of plastic pieces found in the fish stomachs., 
plus two profiles of known plastic for quality check A README text file 
contains the legend of the columns of the three csv files.

The pictures of the plastic particles analysed with ATR-FT-IR can be obtained 
inquiring the data steward.</gco:CharacterString>
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      <gmd:distributionFormat>
        <gmd:MD_Format>
          <gmd:name>
            <gmx:Anchor xlink:type="simple" xlink:href="http://vocab.nerc.ac.uk/collection/M01/current/BIN">Binary</gmx:Anchor>
          </gmd:name>
          <gmd:version gco:nilReason="Unkown" />
        </gmd:MD_Format>
        <gmd:MD_Format>
          <gmd:name>
            <gmx:Anchor xlink:type="simple" xlink:href="http://vocab.nerc.ac.uk/collection/M01/current/DEL">Delimited</gmx:Anchor>
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        </gmd:MD_Format>
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      </gmd:distributionFormat>
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        <gmd:MD_DigitalTransferOptions>
          <gmd:onLine>
            <gmd:CI_OnlineResource>
              <gmd:linkage>
                <gmd:URL>https://data.cefas.co.uk/view/20476</gmd:URL>
              </gmd:linkage>
              <gmd:name>
                <gco:CharacterString>Cefas Data Portal</gco:CharacterString>
              </gmd:name>
              <gmd:function>
                <gmd:CI_OnlineFunctionCode codeList="http://standards.iso.org/iso/19139/resources/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="download">download</gmd:CI_OnlineFunctionCode>
              </gmd:function>
              <gmd:description>
                <gco:CharacterString>The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: Binary download, CSV.</gco:CharacterString>
              </gmd:description>
            </gmd:CI_OnlineResource>
          </gmd:onLine>
        </gmd:MD_DigitalTransferOptions>
      </gmd:transferOptions>
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    <gmd:DQ_DataQuality>
      <gmd:scope>
        <gmd:DQ_Scope>
          <gmd:level>
            <gmd:MD_ScopeCode codeList="http://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_ScopeCode" codeListValue="dataset">dataset</gmd:MD_ScopeCode>
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      </gmd:scope>
      <gmd:report>
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          <gmd:result>
            <gmd:DQ_ConformanceResult>
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                    <gco:CharacterString>INSPIRE Data Specification on Habitats and Biotopes – Technical Guidelines</gco:CharacterString>
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                        <gco:Date>2013-12-10</gco:Date>
                      </gmd:date>
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                <gco:Boolean>false</gco:Boolean>
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                <gco:CharacterString>See the referenced specification</gco:CharacterString>
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      <gmd:report>
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          <gmd:result>
            <gmd:DQ_ConformanceResult>
              <gmd:specification>
                <gmd:CI_Citation>
                  <gmd:title>
                    <gmx:Anchor xlink:type="simple" xlink:href="http://data.europa.eu/eli/reg/2010/1089">Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services</gmx:Anchor>
                  </gmd:title>
                  <gmd:date>
                    <gmd:CI_Date>
                      <gmd:date>
                        <gco:Date>2010-12-08</gco:Date>
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                      <gmd:dateType>
                        <gmd:CI_DateTypeCode codeList="http://standards.iso.org/ittf/PubliclyAvailableStandards/ISO_19139_Schemas/resources/codelist/ML_gmxCodelists.xml#CD_DateTypeCode" codeListValue="publication">publication</gmd:CI_DateTypeCode>
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                  </gmd:date>
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                <gco:CharacterString>See the referenced specification</gco:CharacterString>
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      </gmd:report>
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          <gmd:statement>
            <gco:CharacterString>The Commonwealth Litter Project (CLiP) supported Belize to take action on 
plastics entering the oceans. The assessment of microplastics in biota was 
part of the action plan to define scientific baselines for future monitoring 
purposes and comparison. Samples of anchovies (Engraulis encrasicolus, 178 
specimens), redeye round herring (Etrumeus whiteheadi, 188 specimens) and 
sardines (Sardinops sagax, 227 specimens) were collected during the 36th 
Pelagic recruit survey in 2019 carried out on board of the R.S. Africana by 
the South African Department of Agriculture, Forestry and Fisheries (DAFF). 
Samples were fished in an inshore area along the SA coastline limited by the 
Orange River mouth (West Coast) and George (South Coast) and divided in seven 
areas (strata). In the lab, stomachs were removed, rinsed and chemically 
digested by a 30 percent KOH:NaClO solution with an incubation period of 24 
hours at 40 degrees centigrade. All samples were then filtered, stained with 
Red Nile dye and a digital image was acquired through a microscope. Number of 
particles was counted and corrected for particles count in blank samples. 
Visible particles were analysed using ATR-FT-IR to identify polymer 
composition, comparing their spectrum to a polymers library. ATR-FT-IR is the 
attenuated total reflection Fourier Transform infrared spectroscopy. A Thermo 
Fisher Scientific Nicolet iS5 ATR-FTIR with an OMNIC software (version 
9.9.473) was used and polymers were identified based on the percentage match 
of IR spectra to a polymer library. Only spectra matched greater than 70 
percent were accepted. Spectra were collected in the range 4000 – 650 1/cm at 
a resolution of 4 1/cm.</gco:CharacterString>
          </gmd:statement>
        </gmd:LI_Lineage>
      </gmd:lineage>
    </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
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    <gmx:Anchor xlink:type="simple" xlink:href="http://vocab.nerc.ac.uk/collection/M25/current/MEDIN/">MEDIN</gmx:Anchor>
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    <gco:CharacterString>3.1.1</gco:CharacterString>
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