Genome sequence and gene annotations of the Patagonion toothfish 2019 dataset https://portal.medin.org.uk/portal/start.php?tpc=009_CEFAS4e644fd0-1265-43c1-a759-96a092bd5424 Outputs include the genome of the Patagonian toothfish, including the sequence of the genome and gene annotations and raw sequencing data, which includes the genome and transcriptome sequenced from multiple different tissues, using different sequencing technologies. Patagonian toothfish (*Dissostichus eleginoides*) is an economically and ecologically important fish species in the family Nototheniidae. Juveniles occupy progressively deeper waters as they mature and grow, and adults have been caught as deep as 2500 m, living on or in just above the southern shelves and slopes around the sub-Antarctic islands of the Southern Ocean. As apex predators, they are a key part of the food web, feeding on a variety of prey, including krill, squid, and other fish. Despite its importance, genomic sequence data, which could be used for more accurate dating of the divergence between Patagonian and Antarctic toothfish, or establish whether it shares adaptations to temperature with fish living in more polar or equatorial climes, has so far been limited. 20240501 20240207