{
    "@context": "https://schema.org",
    "@type": "Dataset",
    "@id": "http://portal.medin.org.uk/portal/json-ld/CEFAS26f95f67-6ce8-46d6-8e4a-f5a7879fe28d.jsonld",
    "identifier": "CEFAS26f95f67-6ce8-46d6-8e4a-f5a7879fe28d",
    "url": "http://portal.medin.org.uk/portal/?details&tpc=CEFAS26f95f67-6ce8-46d6-8e4a-f5a7879fe28d",
    "name": "Metabarcoding of pond environmental DNA samples towards development of DNA-based metrics for the assessment of pond condition",
    "description": "Metabarcoding of pond environmental DNA samples towards development of \r\nDNA-based metrics for the assessment of pond condition.\r\n\r\nThis project processed and sequenced water samples (c. 1 L volume) from 61 \r\nfreshwater ponds of varying habitat condition surveyed by Natural England as \r\npart of their England Ecosystem Survey. Additional, equivalent samples (48) \r\nfrom high quality freshwater pond habitats were provided by the Freshwater \r\nHabitats Trust. All samples were collected between May and September 2023, and \r\nsequenced in March 2024.\r\n\r\nAll pond water samples were processed to extract environmental DNA (eDNA), \r\nwhich was sequenced using five metabarcoding markers. This produced species \r\nlists for each sampled pond for five taxonomic groups: vertebrates, \r\ninvertebrates, plants and fungi, microbial eukaryotes, and prokaryotes \r\n(bacteria and archaea). \r\n\r\nAlongside water samples, physicochemical and plant observation data were \r\ncollected for all ponds surveyed, as presented in separate Natural \r\nEngland-commissioned reports. The analysis of pond sequence data alongside \r\nthese data is subject to ongoing metric development.",
    "includedInDataCatalog": {
        "@id": "http://portal.medin.org.uk/portal/json-ld/catalog.jsonld"
    },
    "conditionsOfAccess": [
        "access: noLimitations",
        "use: https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/"
    ],
    "distribution": [
        {
            "@type": "DataDownload",
            "name": "Cefas Data Portal",
            "description": "The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science.",
            "contentUrl": "https://data.cefas.co.uk/view/22282"
        }
    ],
    "temporalCoverage": "2023-05-01/2023-09-01",
    "keywords": [
        {
            "@type": "DefinedTerm",
            "identifier": "https://www.eionet.europa.eu/gemet/en/concept/3489",
            "name": "Freshwater ecosystem"
        },
        {
            "@type": "DefinedTerm",
            "identifier": "https://www.eionet.europa.eu/gemet/en/concept/5333",
            "name": "Molecular biology"
        },
        {
            "@type": "DefinedTerm",
            "identifier": "http://vocab.nerc.ac.uk/collection/P02/current/RBHY/",
            "inDefinedTermSet": "http://vocab.nerc.ac.uk/collection/P02/current/",
            "termCode": "RBHY",
            "name": "Molecular biology parameters"
        },
        {
            "@type": "DefinedTerm",
            "identifier": "http://vocab.nerc.ac.uk/collection/P22/current/sd/",
            "inDefinedTermSet": "http://vocab.nerc.ac.uk/collection/P22/current/",
            "termCode": "sd",
            "name": "Species distribution"
        },
        {
            "@type": "DefinedTerm",
            "identifier": "http://vocab.nerc.ac.uk/collection/P22/current/hb/",
            "inDefinedTermSet": "http://vocab.nerc.ac.uk/collection/P22/current/",
            "termCode": "hb",
            "name": "Habitats and biotopes"
        },
        {
            "@type": "DefinedTerm",
            "identifier": "http://vocab.nerc.ac.uk/collection/N01/current/NDGO0001/",
            "inDefinedTermSet": "http://vocab.nerc.ac.uk/collection/N01/current/",
            "termCode": "NDGO0001",
            "name": "Marine Environmental Data and Information Network"
        },
        {
            "@type": "DefinedTerm",
            "identifier": "http://vocab.nerc.ac.uk/collection/N01/current/NDGO0005/",
            "inDefinedTermSet": "http://vocab.nerc.ac.uk/collection/N01/current/",
            "termCode": "NDGO0005",
            "name": "data.gov.uk"
        }
    ],
    "spatialCoverage": {
        "@type": "Place",
        "geo": {
            "@type": "GeoShape",
            "box": "52.4581 1.73881 52.4595 1.74086"
        },
        "additionalProperty": [
            {
                "@type": "PropertyValue",
                "propertyID": "https://epsg.org/crs_4326/WGS-84.html",
                "value": "WGS 84"
            }
        ]
    },
    "author": [
        {
            "@type": "Organization",
            "name": "Department for Environment, Food and Rural Affairs (DEFRA)",
            "description": "owner",
            "email": "defra.helpline@defra.gov.uk",
            "address": {
                "@type": "PostalAddress"
            }
        }
    ],
    "maintainer": [
        {
            "@type": "Organization",
            "name": "Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)",
            "description": "custodian",
            "email": "data.manager@cefas.co.uk",
            "address": {
                "@type": "PostalAddress",
                "streetAddress": "Cefas Lowestoft Laboratory, Pakefield Road",
                "postalCode": "NR33 0HT",
                "addressLocality": "Lowestoft",
                "addressRegion": "Suffolk",
                "addressCountry": "UK"
            }
        }
    ],
    "producer": [
        {
            "@type": "Organization",
            "name": "Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)",
            "description": "originator",
            "email": "data.manager@cefas.co.uk",
            "address": {
                "@type": "PostalAddress",
                "streetAddress": "Cefas Lowestoft Laboratory, Pakefield Road",
                "postalCode": "NR33 0HT",
                "addressLocality": "Lowestoft",
                "addressRegion": "Suffolk",
                "addressCountry": "UK"
            }
        }
    ],
    "publisher": [
        {
            "@type": "Organization",
            "name": "Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)",
            "description": "pointOfContact",
            "email": "data.manager@cefas.co.uk",
            "address": {
                "@type": "PostalAddress",
                "streetAddress": "Cefas Lowestoft Laboratory, Pakefield Road",
                "postalCode": "NR33 0HT",
                "addressLocality": "Lowestoft",
                "addressRegion": "Suffolk",
                "addressCountry": "UK"
            }
        }
    ],
    "provider": [
        {
            "@type": "Organization",
            "name": "Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)",
            "description": "distributor",
            "email": "data.manager@cefas.co.uk",
            "address": {
                "@type": "PostalAddress",
                "streetAddress": "Cefas Lowestoft Laboratory, Pakefield Road",
                "postalCode": "NR33 0HT",
                "addressLocality": "Lowestoft",
                "addressRegion": "Suffolk",
                "addressCountry": "UK"
            }
        }
    ],
    "creator": [
        {
            "@type": "Organization",
            "name": "Department for Environment, Food and Rural Affairs (DEFRA)",
            "description": "owner",
            "email": "defra.helpline@defra.gov.uk",
            "address": {
                "@type": "PostalAddress"
            }
        }
    ],
    "sdPublisher": {
        "@type": "Organization",
        "url": "https://portal.medin.org.uk/portal/",
        "name": "Marine Environmental Data and Information Network (MEDIN)",
        "description": "The MEDIN portal contains information about 19493 marine datasets from over 600 UK organisations. Metadata are an enduring resource and contact details are publicly available for a long time. \r\n\t\tPlease contact us (enquiries@medin.org.uk) if you find your contact details on the MEDIN portal and do not consent to this. \r\n\t\tYou can now use our GitHub repository to report issues or access related documents. Metadata records are updated daily and were last updated 2026-04-19. Record deletion takes place weekly and the latest occurred 2026-04-12."
    }
}