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These data contain information about the individual accessory pigments of phytoplankton measured by High Pressure Liquid Chromatography (HPLC) from two North Sea International Bottom Trawl Surveys in 2010 and 2011. These have been applied like a 'fingerprint' to identify phytoplankton functional types (PFT's).
Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)
| Other details | ||
| Internal code | Internally assigned metadata identifier | 9080 |
| Title | The title is used to provide a brief and precise description of the dataset such as 'Date', 'Originating organisation/programme', 'Location' and 'Type of survey'. All acronyms and abbreviations should be reproduced in full. | North Sea phytoplankton pigments 2010 to 2011 |
| File Identifier | The File Identifier is a code, preferably a GUID, that is globally unique and remains with the same metadata record even if the record is edited or transferred between portals or tools. | CEFAS0361a083-bc34-428e-b9d0-41c422c2faad |
| Resource Identifier | This is the code assigned by the data owner. | CEFAS18473 |
| Resource type | The resource type will likely be a dataset but could also be a series (collection of datasets with a common specification) or a service. | dataset |
| Start date | This describes the date the resource starts. This may only be the year if month and day are not known | 2010-08-07 |
| End date | This describes the date the resource ends. This may only be the year if month and day are not known | 2011-09-04 |
| Frequency of updates | This describes the frequency with which the resource is modified or updated i.e. a monitoring programme that samples once per year has a frequency that is described as 'annually'. | quarterly |
| Abstract | The abstract provides a clear and brief statement of the content of the resource. | These data contain information about the individual accessory pigments of phytoplankton measured by High Pressure Liquid Chromatography (HPLC) from two North Sea International Bottom Trawl Surveys in 2010 and 2011. These have been applied like a 'fingerprint' to identify phytoplankton functional types (PFT's). |
| Lineage | Lineage includes the background information, history of the sources of data, data quality statements and methods. | 10L water samples were taken from niskin bottles on a rosette fired at various depths, or from the continuous flow ferrybox water supply (approx. 4m water depth) in adverse weather conditions. Water samples (typically 1000ml) were prefiltered with 200 micron gauze to remove larger zooplankton and debris, then filtered through a Whatman GFF filter, and immediately frozen in a -80 degrees C freezer on board. On return to shore, samples were transferred to a -80 degrees C freezer for a storage period of 1-2 months before shipping of samples on dry ice to an accredited HPLC laboratory (DHIWater Quality Institute; Horsholm,Denmark) for chlorophyll a (Chl a) quantification and full accessory pigment analysis. Pigments were extracted adding 6 ml of 95 percent acetone (and an internal standard, vitamin E) to each filter, at 4 degrees C for 20 hours. Samples were then filtered through 0.2 micron Teflon syringe filter into HPLC vials, together with DHI mixed pigments. Buffer and samples were injected on HPLC (Shimadzu LC-10A HPLC system with LC Solution software) in the ratio 5:2 using a pre-treatment program and mixing in the loop before injection. The HPLC method used was the HPL method by Van Heukelem and Thomas (2005). Pigment data from the surface stations were quality data controlled in several steps: first, with an initial comparison of HPLC Chl a against independent measures of chlorophyll fluorescence from the fluorometers on the ship’s FerryBox and CTD system. This step corrected a small number of mislabelled samples. In a second step, anomalies within a sample were detected using methods described by Aiken et al. (2009), e.g. regression of total accessory pigments against Chl a concentration and search for outliers. Diagnostic pigment analysis was then used on the quality controlled data set to relate the composition of specific accessory pigments to the relative contribution of different size classes to the total phytoplankton biomass. The designation of specific accessory pigments to algal taxonomic groups of different size, e.g. fucoxanthin and peridinin for large-cell diatoms and dinoflagellates, has been widely established in the biological oceanographic literature (Uitz et al., 2006, 2008). The equations used to estimate the contribution of pico-phytoplankton (0–2 microns), nano-phytoplankton (2–20 microns) and micro- or net phytoplankton (greater than 20 microns) were later modified by Hirata et al. (2008, 2011) and Brewin et al. (2010). The various methods differ in the degree to which the marker pigments chlorophyll b (Chl b) and 19-hexfucoxanthin (19-hex) are attributed to the three size classes. Here, Chl b and 19-hex were assigned equally to the picophytoplankton and nano-phytoplankton size classes. Pico-phytoplankton are therefore represented by zeaxanthin, Chl b, and 19-hex; nano-phytoplankton are represented by 19-hex, 19-but, alloxanthin, and Chl b; and micro-phytoplankton are represented by fucoxanthin and peridinin. Results are expressed as a proportion of the total Chl a concentration for each station. |
| Related keywords | ||
| Keyword | General subject area(s) associated with the resource, uses multiple controlled vocabularies | Phytoplankton |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Pigments | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Plankton | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Algal bloom | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Chlorophyll pigment concentrations in the water column | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Habitats and biotopes | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Upper epipelagic | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Water column | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Marine Environmental Data and Information Network | |
| Geographical coverage | ||
| North | The northern-most limit of the data resource in decimal degrees | 62.5 |
| East | The eastern-most limit of the data resource in decimal degrees | 14 |
| South | The southern-most limit of the data resource in decimal degrees | 47.5 |
| West | The western-most limit of the data resource in decimal degrees | -13 |
| Responsible organisations | ||
| Role | The point of contact is person or organisation with responsibility for the creation and maintenance of the metadata for the resource. | pointOfContact |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| Role | The originator is the person or organisation who created, collected or produced the resource. | originator |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| Role | The custodian is the person or organisation that accepts responsibility for the resource and ensures appropriate care and maintenance. If a dataset has been lodged with a Data Archive Centre for maintenance then this organisation is be entered here. | custodian |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| Role | The distributor is the person or organisation that distributes the resource. | distributor |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| Role | The owner is the person or organisation that owns the resource. | owner |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Role | The owner is the person or organisation that owns the resource. | owner |
| Organisation name | Department for Environment, Food and Rural Affairs (DEFRA) | |
| Role | The owner is the person or organisation that owns the resource. | owner |
| Organisation name | European Commission | |
| Resource locators | ||
| Locator URL | Web address (URL) that links to the resource | https://data.cefas.co.uk/view/18473 |
| Locator name | Name of the web resource | Cefas Data Portal |
| Dataset constraints | ||
| 20.1 Limitations on Public Access - Access constraints | Any restrictions imposed on accessing the resource such as the need to agree to certain licence conditions. | otherRestrictions |
| 20.2 Limitations on Public Access - Other constraints | https://inspire.ec.europa.eu/metadata-codelist/LimitationsOnPublicAccess/ | |
| 21.1 Conditions for Access and Use - Use constraints | Any restrictions imposed on accessing the resource such as the need to agree to certain licence conditions. | otherRestrictions |
| 21.2 Conditions for Access and Use - Other constraints | http://standards.iso.org/ittf/PubliclyAvailableStandards/ISO_19139_Schemas/resources/codelist/gmxCodelists.xml#MD_RestrictionCode | |
| Version info | ||
| Date of publication | The publication date of the resource or if previously unpublished the date that the resource was made publicly available via the MEDIN network. | 2017-03-17 |
| Date of last revision | The most recent date that the resource was revised. | 2023-05-17 |
| Date of creation | The date that the resource was created. | 2017-03-16 |
| Metadata date | The date when the content of this metadata record was last updated. | 2023-05-17 |
| Metadata standard name | The name of the metadata standard used to create this metadata | MEDIN |
| Metadata standard version | The version of the MEDIN Discovery Metadata Standard used to create the metadata record | 3.1.1 |
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