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2017-2022. Samples were obtained using twin ringnet deployments at set 
stations and subsequently processed back at the lab using ZooScan image 
analysis. Count abundances from semi-automatic image classifications on 
EcoTaxa are then converted into abundances per m3 using estimates of 
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zooplankton results specifically from the PELTIC (Integrated Pelagic ecosystem 
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                <gco:Decimal>52.50</gco:Decimal>
              </gmd:northBoundLatitude>
            </gmd:EX_GeographicBoundingBox>
          </gmd:geographicElement>
        </gmd:EX_Extent>
      </gmd:extent>
      <gmd:extent>
        <gmd:EX_Extent>
          <gmd:temporalElement>
            <gmd:EX_TemporalExtent>
              <gmd:extent>
                <gml:TimePeriod gml:id="0fd3bb0e-876c-4ab9-81dd-ee0afeb10ada">
                  <gml:beginPosition>2017-09-25</gml:beginPosition>
                  <gml:endPosition>2023-03-17</gml:endPosition>
                </gml:TimePeriod>
              </gmd:extent>
            </gmd:EX_TemporalExtent>
          </gmd:temporalElement>
        </gmd:EX_Extent>
      </gmd:extent>
      <gmd:distributionFormat>
        <gmd:MD_Format>
          <gmd:name>
            <gmx:Anchor xlink:type="simple" xlink:href="http://vocab.nerc.ac.uk/collection/M01/current/DEL">Delimited</gmx:Anchor>
          </gmd:name>
          <gmd:version gco:nilReason="Unkown" />
        </gmd:MD_Format>
        <gmd:MD_Format>
          <gmd:name>
            <gmx:Anchor xlink:type="simple" xlink:href="http://vocab.nerc.ac.uk/collection/M01/current/GIS">Geographic Information System</gmx:Anchor>
          </gmd:name>
          <gmd:version gco:nilReason="Unkown" />
        </gmd:MD_Format>
      </gmd:distributionFormat>
    </gmd:MD_DataIdentification>
  </gmd:identificationInfo>
  <gmd:distributionInfo>
    <gmd:MD_Distribution>
      <gmd:distributionFormat>
        <gmd:MD_Format>
          <gmd:name>
            <gco:CharacterString>Unknown</gco:CharacterString>
          </gmd:name>
          <gmd:version gco:nilReason="inapplicable" />
        </gmd:MD_Format>
      </gmd:distributionFormat>
      <gmd:transferOptions>
        <gmd:MD_DigitalTransferOptions>
          <gmd:onLine>
            <gmd:CI_OnlineResource>
              <gmd:linkage>
                <gmd:URL>https://data.cefas.co.uk/view/21495</gmd:URL>
              </gmd:linkage>
              <gmd:name>
                <gco:CharacterString>Cefas Data Portal</gco:CharacterString>
              </gmd:name>
              <gmd:function>
                <gmd:CI_OnlineFunctionCode codeList="http://standards.iso.org/iso/19139/resources/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="download">download</gmd:CI_OnlineFunctionCode>
              </gmd:function>
              <gmd:description>
                <gco:CharacterString>The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: CSV, ESRI Shapefile. The data can also be accessed via the WFS and WMS protocols.</gco:CharacterString>
              </gmd:description>
            </gmd:CI_OnlineResource>
          </gmd:onLine>
        </gmd:MD_DigitalTransferOptions>
      </gmd:transferOptions>
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  </gmd:distributionInfo>
  <gmd:dataQualityInfo>
    <gmd:DQ_DataQuality>
      <gmd:scope>
        <gmd:DQ_Scope>
          <gmd:level>
            <gmd:MD_ScopeCode codeList="http://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_ScopeCode" codeListValue="dataset">dataset</gmd:MD_ScopeCode>
          </gmd:level>
        </gmd:DQ_Scope>
      </gmd:scope>
      <gmd:report>
        <gmd:DQ_DomainConsistency>
          <gmd:result>
            <gmd:DQ_ConformanceResult>
              <gmd:specification>
                <gmd:CI_Citation>
                  <gmd:title>
                    <gco:CharacterString>INSPIRE Data Specification on Species Distribution – Technical Guidelines</gco:CharacterString>
                  </gmd:title>
                  <gmd:date>
                    <gmd:CI_Date>
                      <gmd:date>
                        <gco:Date>2013-12-10</gco:Date>
                      </gmd:date>
                      <gmd:dateType>
                        <gmd:CI_DateTypeCode codeList="http://standards.iso.org/ittf/PubliclyAvailableStandards/ISO_19139_Schemas/resources/codelist/ML_gmxCodelists.xml#CD_DateTypeCode" codeListValue="publication">publication</gmd:CI_DateTypeCode>
                      </gmd:dateType>
                    </gmd:CI_Date>
                  </gmd:date>
                </gmd:CI_Citation>
              </gmd:specification>
              <gmd:pass>
                <gco:Boolean>false</gco:Boolean>
              </gmd:pass>
              <gmd:explanation>
                <gco:CharacterString>See the referenced specification</gco:CharacterString>
              </gmd:explanation>
            </gmd:DQ_ConformanceResult>
          </gmd:result>
        </gmd:DQ_DomainConsistency>
      </gmd:report>
      <gmd:report>
        <gmd:DQ_DomainConsistency>
          <gmd:result>
            <gmd:DQ_ConformanceResult>
              <gmd:specification>
                <gmd:CI_Citation>
                  <gmd:title>
                    <gmx:Anchor xlink:type="simple" xlink:href="http://data.europa.eu/eli/reg/2010/1089">Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services</gmx:Anchor>
                  </gmd:title>
                  <gmd:date>
                    <gmd:CI_Date>
                      <gmd:date>
                        <gco:Date>2010-12-08</gco:Date>
                      </gmd:date>
                      <gmd:dateType>
                        <gmd:CI_DateTypeCode codeList="http://standards.iso.org/ittf/PubliclyAvailableStandards/ISO_19139_Schemas/resources/codelist/ML_gmxCodelists.xml#CD_DateTypeCode" codeListValue="publication">publication</gmd:CI_DateTypeCode>
                      </gmd:dateType>
                    </gmd:CI_Date>
                  </gmd:date>
                </gmd:CI_Citation>
              </gmd:specification>
              <gmd:pass>
                <gco:Boolean>true</gco:Boolean>
              </gmd:pass>
              <gmd:explanation>
                <gco:CharacterString>See the referenced specification</gco:CharacterString>
              </gmd:explanation>
            </gmd:DQ_ConformanceResult>
          </gmd:result>
        </gmd:DQ_DomainConsistency>
      </gmd:report>
      <gmd:lineage>
        <gmd:LI_Lineage>
          <gmd:statement>
            <gco:CharacterString>Since autumn 2017, samples have continued to be collected with twin ringnet 
deployments and KC flowmeter attachments onboard the RV Cefas Endeavour, with 
image analysis conducted on those obtained using an 80µm ringnet of 0.5m 
diameter. Deployments are made at set station locations along transect lines 
which continue to span the area between the Bristol Channel to the south of 
Wales and eastern Celtic Sea to the western side of the English Channel. In 
addition to this, the surveys expanded their focus from purely UK territorial 
waters around the Cornish Peninsula to include French waters off the western 
English Channel. In 2020, this was extended further to include Cardigan Bay to 
the west of Wales. The transect lines stations have been located within have 
been designed with their spacing and orientation across the prevailing 
bathymetry of the area.

In autumn 2022, a restricted survey period meant that only the English Channel 
area was sampled and a subsequent survey took place in March 2023 which 
focused on the Cardigan Bay area (CEND 3b/23).

Preserved samples were sent back to the Plankton Lab at the Cefas Lowestoft 
Laboratory and thereafter prepped for imaging using ZooScan. A minimum 
threshold of 200 zooplankton specimens were aimed for in the images where 
possible from each sample, with rescans conducted as necessary. Object images 
processed from the sample were subsequently imported into the project image 
library within the EcoTaxa web application.

For samples obtained in the CEND 3b/23 survey in March 2023, the method was 
altered so that samples were separated into a 1mm+ size fraction (d1) and 
80µm-1mm size fraction (d2). Both the d1 and d2 components were separately 
diluted if necessary and imaged as above, to enable a more accurate 
representation of larger and rarer zooplankton taxa.

Once uploaded into EcoTaxa, scanned objects from each sample were predicted 
into taxa categories with the provision of a learning set to aid automatic 
image sorting using Random Forest algorithms. These predicted classifications 
were validated afterward by an experienced zooplankton taxonomist and the 
metadata downloaded with the taxa counts. Filtered volume estimates were 
obtained based on calculations using flowmeter rotor revolution counts 
recorded for each station. Objects captured in images were separated into 
zooplankton taxa and detritus groups and abundances estimated in terms of per 
m3, using counts and filtered volume and raising factor calculations based 
on the sample fractions used. An agreed subset of the data may be selected for 
quality control checks by a second experienced taxonomist, ensuring quality 
control in the image classifications.</gco:CharacterString>
          </gmd:statement>
        </gmd:LI_Lineage>
      </gmd:lineage>
    </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
  <gmd:metadataStandardName>
    <gmx:Anchor xlink:type="simple" xlink:href="http://vocab.nerc.ac.uk/collection/M25/current/MEDIN/">MEDIN</gmx:Anchor>
  </gmd:metadataStandardName>
  <gmd:metadataStandardVersion>
    <gco:CharacterString>3.1.1</gco:CharacterString>
  </gmd:metadataStandardVersion>
</gmd:MD_Metadata>