CEFAS2c27ff32-858c-470e-9a52-01956b49e63c English dataset Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk pointOfContact 2023-03-01T04:14:34 EPSG Geodetic Parameter Registry 2022-01-07 revision WGS 84 Dietary metabarcoding reads from stomachs of fish found in the Irish Sea, Celtic Sea, English Channel, and North Sea from 2015 to 2016 2023-03-01 publication 2023-03-01 revision 2023-02-24 creation CEFAS21645 https://data.cefas.co.uk Raw sequences produced in 2018 from the stomach samples of lemon sole (n=16), herring (n=36), whiting (n=27), plaice (n=24), haddock (n=15), saithe (n=20), horse mackerel (n=15), grey gurnard(n=15), and mackerel (n=38). Samples were obtained from the North Sea, Eastern channel, and Irish and Celtic Seas. The sequences were analysed using a MiSeq benchtop sequencer. Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk originator Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk custodian Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk distributor Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) data.manager@cefas.co.uk owner notPlanned Molecular biology GEMET, version 1.0 2008-06-01 publication Species distribution GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Inapplicable SeaVoX Vertical Co-ordinate Coverages 2010-05-18 revision Marine Environmental Data and Information Network MEDIN metadata record availability 2012-01-11 publication data.gov.uk MEDIN metadata record availability 2012-01-11 publication otherRestrictions noLimitations otherRestrictions **Publicly available data licensed under the Open Government Licence v3.0 (`https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/)`_ – Please note the applicable attribution statement is as follows: ‘includes Crown copyright, Cefas 2023** .. _`https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/)`: https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ English biota -11.00 5.00 49.00 60.50 2018-01 2023-03-01 Binary Unknown https://data.cefas.co.uk/view/21645 Cefas Data Portal download The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: Binary download. dataset INSPIRE Data Specification on Species Distribution – Technical Guidelines 2013-12-10 publication false See the referenced specification Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 publication true See the referenced specification Full details are available in 5.3 of `*Resolving the role of jellyfish in marine food webs*`_. In brief: fish stomach samples were obtained from North Sea International Beam Trawl Survey (IBTS) (8 August – 6th September 2016), Eastern channel beam trawl (17th– 30th July 2016), and the Poseidon (3rd -21stOctober 2015) research cruises were collected aboard the RV Cefas Endeavour. Samples from the Celtic sea herring acoustic survey (CSHAS) (7th-27th October 2016) were collected aboard the RV Celtic explorer. Stomachs were removed and the contents DNA was extracted by salt for Poseidon samples, and CTAB DNA extraction for all other samples. 1st round PCRS were conducted using two sets of primers: ILF_ProSSU3 (18s) and Uni-Minibar (COI). Second round PCR attached unique nucleotide indexes. Samples were sequenced on MiSeq and demultiplexed at Sheffield diagnostic genetics services. .. _`*resolving the role of jellyfish in marine food webs*`: https://ueaeprints.uea.ac.uk/id/eprint/69971/1/2019LambPPhD.pdf MEDIN 3.1.1