CEFAS5db0317a-6080-44d8-8c65-0cad0853c646 English dataset Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk pointOfContact 2024-07-12T12:52:38 EPSG Geodetic Parameter Registry 2022-01-07 revision WGS 84 Northwest European shelf seas - Phytoplankton pigments - 2010 to 2019 2013-10-17 publication 2024-07-12 revision 2011-10-07 creation CEFAS53 https://data.cefas.co.uk These data contain concentrations in micrograms per litre (ug/L) of the individual accessory pigments of phytoplankton measured by High Pressure Liquid Chromatography (HPLC). Pigment samples were taken primarily on Cefas fisheries and SmartBuoy cruises in the North Sea and Celtic Seas, and sent to DHI laboratory in Denmark for HPLC analysis. Data was then sent back to Cefas and matched with metadata and quality controlled. Chlorophyll concentrations from this dataset have a variety of uses: for assessment of concentrations for purposes such as eutrophication assessments, validation of remote sensing derived chlorophyll estimates, as a proxy for phytoplankton biomass, or to calibrate fluorometers. Pigment concentrations can be applied like a fingerprint to derive phytoplankton functional types and give information about the phytoplankton community. Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk originator Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk custodian Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk distributor Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) data.manager@cefas.co.uk owner Department for Environment, Food and Rural Affairs (DEFRA) defra.helpline@defra.gov.uk owner European Commission info@publications.europa.eu owner annually Chlorophyll pigment concentrations in the water column SeaDataNet P02 parameter discovery vocabulary 2011-03-25 revision Phytoplankton Pigments SeaDataNet P03 parameter discovery vocabulary 2011-03-25 revision Remote Sensing Productivity GEMET, version 1.0 2008-06-01 publication Habitats and biotopes Oceanographic geographical features GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Upper epipelagic Water column SeaVoX Vertical Co-ordinate Coverages 2010-05-18 revision Marine Environmental Data and Information Network MEDIN metadata record availability 2012-01-11 publication data.gov.uk MEDIN metadata record availability 2012-01-11 publication otherRestrictions noLimitations otherRestrictions **Publicly available data licensed under the Open Government Licence v3.0 (`https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/)`_ – Please note the applicable attribution statement is as follows: ‘includes Crown copyright, Cefas [2019]'** .. _`https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/)`: https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ English environment oceans -13.00 14.00 47.50 62.50 2010-01-08 2019-10-27 Delimited Geographic Information System Unknown https://data.cefas.co.uk/view/53 Cefas Data Portal download The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: CSV, ESRI Shapefile. The data can also be accessed via the WFS and WMS protocols. dataset INSPIRE Data Specification on Habitats and Biotopes – Technical Guidelines 2013-12-10 publication false See the referenced specification INSPIRE Data Specification on Oceanographic geographical features – Technical Guidelines 2013-12-10 publication false See the referenced specification Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 publication true See the referenced specification 2 litre water samples were collected on board the RV Cefas Endeavour from the continuous flow ferrybox water supply (4m depth), or from Niskin bottles on a rosette fired at particular depths. Water samples were filtered through a Whatman GFF filter, and immediately frozen in liquid nitrogen (2010-2012) or in a -80°C freezer on board (other years). Pigments were stored at -80°C for 1-4 months, before being selected for analysis, then transferred to the analysis laboratory (DHI; Horsholm, Denmark) using dry ice. Pigments were extracted adding 6 ml of 95% acetone (and an internal standard, vitamin E) to each filter. The samples were sonicated in an ice-cold sonication bath for 10 min and extracted at 4 °C for 20 hours. The samples were then filtered through 0.2 µm Teflon syringe filter into HPLC vials. Buffer and samples were injected on HPLC (Shimadzu LC-10A HPLC system with LC Solution software) in the ratio 5:2 using a pre-treatment program and mixing in the loop before injection (Schlüter et al 2016). The HPLC method used was the Van Heukelem & Thomas (2005) method where the gradient was adjusted to optimise the resolution of the pigments. The raw data were stored in an MS Access database. Quality control was performed after Aiken et al 2009. Samples were removed if they had insufficient chlorophyll (<0.04ug/L), a ratio of above 0.5 of degradation pigments (chlorophyllide + pheophorbide + pheophytin) to chlorophyll a, or if there was a difference between total chlorophyll a (TChla) and accessory pigments (AP) of more than 40% of the total pigments. Additionally, where at least 5 samples were collected for a survey, total chlorophyll a was regressed against total accessory pigments, and the data from the survey was rejected if the regression was poor (r2<0.75, slope <0.65 or <1.4). MEDIN 3.1.1