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from CTD casts, microplastic abundance in water sampled by microplastic pump, 
microplastic abundance in sediment and particulate size analysis (PSA) results 
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blank values. A series of atmospheric blanks was also obtained leaving a jar 
open during sampling operation and microplastics abundances are reported. The 
csv second file (South_Africa_Port_Durban_Microplastics_FTIR) contains the 
profiles of the ATR-FT-IR spectrum analysis of plastic pieces found in the 
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        </gmd:MD_Format>
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            <gmx:Anchor xlink:type="simple" xlink:href="http://vocab.nerc.ac.uk/collection/M01/current/DEL">Delimited</gmx:Anchor>
          </gmd:name>
          <gmd:version gco:nilReason="Unkown" />
        </gmd:MD_Format>
        <gmd:MD_Format>
          <gmd:name>
            <gmx:Anchor xlink:type="simple" xlink:href="http://vocab.nerc.ac.uk/collection/M01/current/GIS">Geographic Information System</gmx:Anchor>
          </gmd:name>
          <gmd:version gco:nilReason="Unkown" />
        </gmd:MD_Format>
      </gmd:distributionFormat>
    </gmd:MD_DataIdentification>
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        <gmd:MD_DigitalTransferOptions>
          <gmd:onLine>
            <gmd:CI_OnlineResource>
              <gmd:linkage>
                <gmd:URL>https://data.cefas.co.uk/view/20510</gmd:URL>
              </gmd:linkage>
              <gmd:name>
                <gco:CharacterString>Cefas Data Portal</gco:CharacterString>
              </gmd:name>
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                <gmd:CI_OnlineFunctionCode codeList="http://standards.iso.org/iso/19139/resources/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="download">download</gmd:CI_OnlineFunctionCode>
              </gmd:function>
              <gmd:description>
                <gco:CharacterString>The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: Binary download, CSV, ESRI Shapefile. The data can also be accessed via the WFS and WMS protocols.</gco:CharacterString>
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            </gmd:CI_OnlineResource>
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      <gmd:scope>
        <gmd:DQ_Scope>
          <gmd:level>
            <gmd:MD_ScopeCode codeList="http://standards.iso.org/iso/19139/resources/gmxCodelists.xml#MD_ScopeCode" codeListValue="dataset">dataset</gmd:MD_ScopeCode>
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          <gmd:result>
            <gmd:DQ_ConformanceResult>
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                <gmd:CI_Citation>
                  <gmd:title>
                    <gco:CharacterString>INSPIRE Data Specification on Habitats and Biotopes – Technical Guidelines</gco:CharacterString>
                  </gmd:title>
                  <gmd:date>
                    <gmd:CI_Date>
                      <gmd:date>
                        <gco:Date>2013-12-10</gco:Date>
                      </gmd:date>
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                        <gmd:CI_DateTypeCode codeList="http://standards.iso.org/ittf/PubliclyAvailableStandards/ISO_19139_Schemas/resources/codelist/ML_gmxCodelists.xml#CD_DateTypeCode" codeListValue="publication">publication</gmd:CI_DateTypeCode>
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          <gmd:result>
            <gmd:DQ_ConformanceResult>
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                    <gmx:Anchor xlink:type="simple" xlink:href="http://data.europa.eu/eli/reg/2010/1089">Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services</gmx:Anchor>
                  </gmd:title>
                  <gmd:date>
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                        <gco:Date>2010-12-08</gco:Date>
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          <gmd:statement>
            <gco:CharacterString>The Commonwealth Litter Project (CLiP) supported South Africa to take action 
on plastics entering the oceans. The abundance of microplastics was 
investigated within The Port of Durban in the Durban Harbour (east coast of 
South Africa).

A handheld CTD multi-channel logger (RBRconcerto3 C.T.D++, RBR Ltd., Canada), 
with attached optical backscatter turbidity (STM, Seapoint Sensors Inc, USA), 
was used to measure temperature, salinity and turbidity. Microplastics in 
water were sampled using a microplastic pump (KC Denmark Plankton Pump for 
Microplastics, Model 23.580) deployed through a crane from the quayside. The 
pump filtered 2000lt of water several sieves: 5mm, 500µm, 300µm, 200µm and 
100µm . Sediment samples were collected at each microplastic pump site for 
sediment particle size analysis (PSA) and sediment microplastic analysis. 
Also, 15 additional sediment samples were taken from a boat on channels and 
areas of deposition. Five additional samples were taken in the harbour and 
surrounds where substrate was suitable. 

Each sediment and water sample was transferred to glass collecting pots. The 
sediment samples were dried at 50 degrees Celsius and, once dried, 5g 
triplicates were taken from a homogenised sample and underwent density 
separation before being chemically digested with a 30 percent KOH:NaClO 
solution. Each sample was then incubated for 72 hours before filtration 
Identification of the extracted microplastics was carried out using the 
fluorescence tagging of polymers using Nile Red coupled with digital imaging 
(Maes et al., 2017). 

For each sediment sample, a PSA was carried-out to relate abundance of 
microplastics to sediment type. Samples were freezed and then underwent PSA, 
based on a modified NMBAQC protocol from Mason (2011) for fast PSA screening 
based on wet splitting into silt/clay (&lt; 63 µm), sand (63 µm – 4 mm) and 
gravel (&gt; 4 mm) fractions. Once dry, samples were weighed, and the proportion 
of each fraction was calculated.

Surface water samples were inspected for any suspected anthropogenic 
particles. Mesoplastics were manually removed, dried and characterised with 
ATR-FTIR. Samples with low to no organic content were filtered and stained 
with Nile Red before imaging and particle counting (Maes et al., 2017). For 
samples with high organic content, sieve rinse water was digested with a 30% 
KOH:NaClO solution with 24 hours incubation. Visible particles were analysed 
using ATR-FT-IR to identify polymer composition, comparing their spectrum to a 
polymers library. ATR-FTIR is the attenuated total reflection Fourier 
Transform infrared spectroscopy. A Thermo Fisher Scientific Nicolet iS5 
ATR-FTIR with an OMNIC software (version 9.9.473) was used and polymers were 
identified based on the percentage match of IR spectra to a polymer library. 
Only spectra matched greater than 70 percent were accepted. Spectra were 
collected in the range 4000 – 650 1/cm at a resolution of 4 1/cm.</gco:CharacterString>
          </gmd:statement>
        </gmd:LI_Lineage>
      </gmd:lineage>
    </gmd:DQ_DataQuality>
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    <gmx:Anchor xlink:type="simple" xlink:href="http://vocab.nerc.ac.uk/collection/M25/current/MEDIN/">MEDIN</gmx:Anchor>
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  <gmd:metadataStandardVersion>
    <gco:CharacterString>3.1.1</gco:CharacterString>
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