CEFASb721a5bb-edaa-4754-b8a5-d7c9f1a350bd English dataset Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk pointOfContact 2025-04-11T12:45:12 EPSG Geodetic Parameter Registry 2022-01-07 revision OSGB 1936 / British National Grid Norovirus, ammonia and orthophosphate in wastewater samples collected from across England 2021 - 2022 2024-01-26 publication 2025-04-11 revision 2023-12-06 creation CEFAS21753 https://data.cefas.co.uk The **sewage treatment works****dataset** includes norovirus levels, ammonia concentrations and orthophosphate concentrations in wastewater from 3,232 samples and their associated metadata collected for the Environmental Monitoring for Health Protection (EMHP) programme between May 2021 and March 2022 from the untreated influent of 152 sewage treatment works across England. The samples are a subset of samples that were originally collected and analysed for SARS-CoV-2. The **network dataset **includes all of the norovirus levels, ammonia concentrations and orthophosphate concentrations in wastewater from 220 samples and their associated metadata collected for the Environmental Monitoring for Health Protection (EMHP) programme between May 2021 and March 2022 from 11 sewerage network sites across England. "NETWORK" sites were located further upstream of the sewershed than the sewage treatment works (STWs). Network sites were typically used during the COVID-19 pandemic to get better spatially resolved data than was possible using sewage works alone. Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk originator Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk custodian Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk distributor Department for Environment, Food and Rural Affairs (DEFRA) defra.helpline@defra.gov.uk owner notPlanned Virus abundance in water bodies SeaDataNet P02 parameter discovery vocabulary 2011-03-25 revision Chemical in the environment GEMET, version 1.0 2008-06-01 publication Habitats and biotopes GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Inapplicable SeaVoX Vertical Co-ordinate Coverages 2010-05-18 revision Marine Environmental Data and Information Network MEDIN metadata record availability 2012-01-11 publication data.gov.uk MEDIN metadata record availability 2012-01-11 publication otherRestrictions noLimitations otherRestrictions Public data - no limitations to reuse English environment -6.50 2.50 49.50 56.25 2021-05-27 2022-03-31 Binary Delimited Unknown https://data.cefas.co.uk/view/21753 Cefas Data Portal download The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: Binary download, CSV. dataset INSPIRE Data Specification on Habitats and Biotopes – Technical Guidelines 2013-12-10 publication false See the referenced specification Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 publication true See the referenced specification The samples were originally received by Environment Agency, and were taken by the companies who owned the assets. The samples were spiked with an extraneous control virus (phi6) to act as a quality control before the viruses were concentrated by ammonium sulphate precipitation and nucleic acid extraction using the NucliSENS reagents with Kingfisher robot. The nucleic acids were first used to quantify SARS-CoV-2 in the samples before being stored at -80°C. The norovirus data were generated from the nucleic acid extracts by the Environment Agency at Starcross laboratory based in Exeter on behalf of Cefas. Norovirus concentrations were measured using a duplex RT-qPCR duplex assay. The duplex assay measures norovirus genogroup I (GI) and genogroup II (GII) separately. Norovirus data were converted from genome copies per reaction to genome copies per litre, and then further normalised for flow and population size for the sewershed. Flow was estimated using ammonia and orthophosphate concentrations for each sample. Human population was estimated using ONS (Office of National Statistics)** **mid-2019 estimates based on data from the 2011 census. Ammonia and orthophosphate concentrations (mg/l) were measured in the samples according to the Environment Agency’s in-house methods. These methods were based on the relevant Standing Committee of Analysts methods (SCA). MEDIN 3.1.1