CEFASdb729d01-5ce7-466e-9a83-81ccde2108b6 English dataset Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk pointOfContact 2024-10-03T08:57:34 EPSG Geodetic Parameter Registry 2022-01-07 revision WGS 84 Molecular characterization of an Endozoicomonas-like species associated with mass mortalities of king scallop Pecten maximus L. in United Kingdom Metagenomic sequencing of King scallop (Pecten maximus) gills infected with Endozoicomonas sp.-like organisms 2017-05-19 publication 2024-10-03 revision 2017-05-05 creation CEFAS18522 https://data.cefas.co.uk The king scallop Pecten maximus L. is the fastest growing fishery in the UK and currently the second most valuable. Mass mortality events in scallops have been reported associated with rickettsia-like organisms (RLOs) world-wide, however the causative agents were uncharacterised. In May 2013 and 2014 two mass mortality events affecting king scallops were recorded in the Lyme Bay Marine Protected Area (MPA) South West England. Histopathological examination showed gill epithelial tissue infected with RLO at varying intensity. Ultrastructural examination confirmed the intracellular location in affected epithelial cells. Partial 16S rDNA sequences of a putative RLO obtained from infected king scallop gill samples collected from both mortality events were identical and had 99.4% identity to 16S rDNA sequences obtained from Candidatus endonucleobacter bathymodioli and 95% with Endozoicomonas spp. In situ hybridisation assays using 16S rDNA probes confirmed the presence of the sequenced RLO gene in the gill tissues. Additional DNA sequences of the bacterium were obtained using high-throughput (Illumina) sequencing and bioinformatic analysis identified over 1000 genes with high similarity, ranging from 77-87% identity, to genes from Endozoicomonas spp. Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk originator Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk custodian Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) Cefas Lowestoft Laboratory Pakefield Road Lowestoft Suffolk NR33 0HT UK data.manager@cefas.co.uk distributor notPlanned Pathology Analysis Molecular biology Sample GEMET, version 1.0 2008-06-01 publication Disease, damage and mortality SeaDataNet P03 parameter discovery vocabulary 2011-03-25 revision Species distribution GEMET - INSPIRE themes, version 1.0 2008-06-01 publication Unknown SeaVoX Vertical Co-ordinate Coverages 2010-05-18 revision Marine Environmental Data and Information Network MEDIN metadata record availability 2012-01-11 publication data.gov.uk MEDIN metadata record availability 2012-01-11 publication otherRestrictions noLimitations otherRestrictions Publicly available data licensed under the Open Government Licence v3.0 ( `https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/)`_ – Please note the applicable attribution statement is as follows: ‘includes Crown copyright, Cefas 2017' .. _`https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/)`: https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ English biota -3.50 -2.00 50.25 50.75 2015-05 2015-05 Binary Unknown https://data.cefas.co.uk/view/18522 Cefas Data Portal download The Cefas Data Portal contains metadata records and data sets available to download and connect to in support of our commitment to open science. Data is available in the following formats: Binary download. dataset INSPIRE Data Specification on Species Distribution – Technical Guidelines 2013-12-10 publication false See the referenced specification Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 publication true See the referenced specification Specific PCR assays were developed and applied to screen for the presence of 16S rDNA RLO sequences in king scallop gill tissues collected in the Lyme Bay MPA during 2015. There was 100% prevalence of the RLO in these gill tissues and the 16S rDNA sequences identified were identical to the sequence found during the previous mortality event. MEDIN 3.1.1