acf1351c-eaed-4ec6-8da5-2b159ba9d926 English nonGeographicDataset 3bdb38f7-495c-4839-9371-2dcaebedfb37 Natalie Hold Bangor University School of Ocean Sciences n.hold@bangor.ac.uk pointOfContact 2025-06-11 MEDIN 1.0 Reconstructing temperature profiles for the common whelk, Buccinum undatum, using stable isotopes Using stable isotopes to create temperature reconstructions and estimate growth rate of the common whelk, Buccinum undatum Stable isotopes of whelk shells 2025-06-30 publication 2025-02-28 revision 2024-07-10 creation BU11403 https://bangor.ac.uk Stable isotope data (δ18O) collected from the shells of the common whelk, Buccinum undatum, from three sites (Shetland, Nefyn and Jersey) for the purpose of estimating in-situ temperatures experienced during shell calcification, from early as 2013. These estimations allowed the reconstruction of temperature profiles and calculation of growth rates over 6 month periods. Charlotte Colvin Bangor University School of Ocean Sciences Marine Biology Lecturer c.n.colvin@bangor.ac.uk owner Natalie Hold Bangor University School of Ocean Sciences Lecturer in Sustainable Marine Fisheries n.hold@bangor.ac.uk custodian Charlotte Colvin Bangor University School of Ocean Sciences Marine Biology Lecturer c.n.colvin@bangor.ac.uk originator Natalie Hold Bangor University School of Ocean Sciences Lecturer in Sustainable Marine Fisheries n.hold@bangor.ac.uk distributor notPlanned Marine Environmental Data and Information Network MEDIN metadata record availability 2025-06-25 revision Shellfish morphology, age and physiology Stable isotopes in biota SeaDataNet Parameter Discovery Vocabulary 2021-09-28 revision otherRestrictions Data currently inaccessible as project is yet to be completed. Contact n.hold@bangor.ac.uk for enquiries regarding data access. otherRestrictions Data currently inaccessible as project is yet to be completed. Contact n.hold@bangor.ac.uk for enquiries regarding data access and use. English 8-bit variable size UCS Transfer Format, based on ISO/IEC 10646 biota SeaVox salt and freshwater body gazetteer 2021-01-06 creation inapplicable 2023-04-21 2025-03-31 Database nonGeographicDataset nonGeographicDataset Commission Regulation (EU) No 1089/2010 of 23 November 2010 implementing Directive 2007/2/EC of the European Parliament and of the Council as regards interoperability of spatial data sets and services 2010-12-08 publication Does not have a spatial element. Data obtained from laboratory processes. false Twenty three shells of variable size and of both sexes were selected from the three study sites (Shetland, Nefyn and Jersey, latitude and longitude of fishing unknown). Chosen shells showed little shell damage, to prevent sampling across repaired (more recent) shell growth and maximise the number of growth cycles presented. B. undatum shells were prepared by thoroughly cleaning to remove surface debris and organic material, followed by dripping in 5% HCl to loosen remaining contaminants, followed by a scrub with a Decon90 mix with water, and then a final wash with deionised water. Shells dried in an oven at 40 °C for around 3 hours. Sequential samples were taken from the shell using a Dremel Lite 7760 Cordless Rotary Tool with a diamond point drill tip (1 mm diameter). Shallow indentations using the drill were consecutively created in the outer prismatic layer, along a single shell striation, from the lip backwards. This ensured that samples were representative of the time at shell formation. The produced powder was collected on weighing paper positioned beneath the shell. To prevent cross-contamination, the weighing paper was replaced, and the drill tip cleaned after each sample. Powder was transferred into labelled 1.5 mL Micro Centrifuge Tubes with caps and placed in an MTC™ Bio Fraction Collector Tube Rack. Approximately 100 - 250 ug of whelk material was used for isotope analysis using either an IsoPrime 100 dual inlet or an IsoPrime isoFLOW mass spectrometer at the British Geological Survey. Samples were loaded into glass vials and sealed with septa. The automated systems evacuate the vials and deliver anhydrous phosphoric acid to the samples at a set temperature. Isotope values (δ13C, δ18O) are reported as per mille (‰) deviations of the isotopic ratios (13C/12C, 18O/16O) calculated to the VPDB scale using a within-run laboratory standard calibrated against NBS-19. The aragonite-acid fractionation factor applied to the gas values is 1.00854. The Craig correction is also applied to account for δ17O. The average analytical reproducibility of the standard calcite (KCM) is <0.1‰ for δ13C and δ18O. During data preparation, interpolation was carried out for samples which could not be processed due to either a sampling or analytical error. Data analyses was conducted in R (4.3.3) and a species-specific paleotemperature equation and site-specific water isotope (δ18Ow) values (Hollyman et al., 2020) were used to estimate temperatures from δ18Os (shell) isotope data. Dates of thermal maxima and minima, derived from hourly sea bottom temperature data for each location, were assigned to each temperature reconstruction. Dates were then interpolated for each 6 month period, allowing analyses of growth rates for individual whelk. Data analyses and project completion is still in process. Contact n.hold@bangor.ac.uk for further information regarding data use and accessibility.