© OpenStreetMap contributors
- Add External Layer
- Upload Shapefile
- Layer Tutorial
- Close
Use the checkbox () to show or hide a layer.
Use the radio buttons () to select a layer to use with the identify function.
- Layers
- Opacity
Available layers
(ctrl+c)
Search for marine data across UK organisations
- API
- How-To
- About
- Contact MEDIN
- Share
Metadata: Molecular characterization of an Endozoicomonas-like species associated with mass mortalities of king scallop Pecten maximus L. in United Kingdom
Abstract:
The king scallop Pecten maximus L. is the fastest growing fishery in the UK and currently the second most valuable. Mass mortality events in scallops have been reported associated with rickettsia-like organisms (RLOs) world-wide, however the causative agents were uncharacterised. In May 2013 and 2014 two mass mortality events affecting king scallops were recorded in the Lyme Bay Marine Protected Area (MPA) South West England. Histopathological examination showed gill epithelial tissue infected with RLO at varying intensity. Ultrastructural examination confirmed the intracellular location in affected epithelial cells. Partial 16S rDNA sequences of a putative RLO obtained from infected king scallop gill samples collected from both mortality events were identical and had 99.4% identity to 16S rDNA sequences obtained from Candidatus endonucleobacter bathymodioli and 95% with Endozoicomonas spp. In situ hybridisation assays using 16S rDNA probes confirmed the presence of the sequenced RLO gene in the gill tissues. Additional DNA sequences of the bacterium were obtained using high-throughput (Illumina) sequencing and bioinformatic analysis identified over 1000 genes with high similarity, ranging from 77-87% identity, to genes from Endozoicomonas spp.
Data holder:
Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS)
| Other details | ||
| Internal code | Internally assigned metadata identifier | 10907 |
| Title | The title is used to provide a brief and precise description of the dataset such as 'Date', 'Originating organisation/programme', 'Location' and 'Type of survey'. All acronyms and abbreviations should be reproduced in full. | Molecular characterization of an Endozoicomonas-like species associated with mass mortalities of king scallop Pecten maximus L. in United Kingdom |
| Alternative title | The purpose of alternative title is to record any additional names by which the dataset may be known. | Metagenomic sequencing of King scallop (Pecten maximus) gills infected with Endozoicomonas sp.-like organisms |
| File Identifier | The File Identifier is a code, preferably a GUID, that is globally unique and remains with the same metadata record even if the record is edited or transferred between portals or tools. | CEFASdb729d01-5ce7-466e-9a83-81ccde2108b6 |
| Resource Identifier | This is the code assigned by the data owner. | CEFAS18522 |
| Resource type | The resource type will likely be a dataset but could also be a series (collection of datasets with a common specification) or a service. | dataset |
| Start date | This describes the date the resource starts. This may only be the year if month and day are not known | 2026-04-17 |
| End date | This describes the date the resource ends. This may only be the year if month and day are not known | 2026-04-17 |
| Frequency of updates | This describes the frequency with which the resource is modified or updated i.e. a monitoring programme that samples once per year has a frequency that is described as 'annually'. | notPlanned |
| Abstract | The abstract provides a clear and brief statement of the content of the resource. | The king scallop Pecten maximus L. is the fastest growing fishery in the UK and currently the second most valuable. Mass mortality events in scallops have been reported associated with rickettsia-like organisms (RLOs) world-wide, however the causative agents were uncharacterised. In May 2013 and 2014 two mass mortality events affecting king scallops were recorded in the Lyme Bay Marine Protected Area (MPA) South West England. Histopathological examination showed gill epithelial tissue infected with RLO at varying intensity. Ultrastructural examination confirmed the intracellular location in affected epithelial cells. Partial 16S rDNA sequences of a putative RLO obtained from infected king scallop gill samples collected from both mortality events were identical and had 99.4% identity to 16S rDNA sequences obtained from Candidatus endonucleobacter bathymodioli and 95% with Endozoicomonas spp. In situ hybridisation assays using 16S rDNA probes confirmed the presence of the sequenced RLO gene in the gill tissues. Additional DNA sequences of the bacterium were obtained using high-throughput (Illumina) sequencing and bioinformatic analysis identified over 1000 genes with high similarity, ranging from 77-87% identity, to genes from Endozoicomonas spp. |
| Lineage | Lineage includes the background information, history of the sources of data, data quality statements and methods. | Specific PCR assays were developed and applied to screen for the presence of 16S rDNA RLO sequences in king scallop gill tissues collected in the Lyme Bay MPA during 2015. There was 100% prevalence of the RLO in these gill tissues and the 16S rDNA sequences identified were identical to the sequence found during the previous mortality event. |
| Related keywords | ||
| Keyword | General subject area(s) associated with the resource, uses multiple controlled vocabularies | Pathology |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Analysis | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Molecular biology | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Sample | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Disease, damage and mortality | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Species distribution | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Unknown | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | Marine Environmental Data and Information Network | |
| General subject area(s) associated with the resource, uses multiple controlled vocabularies | data.gov.uk | |
| Geographical coverage | ||
| North | The northern-most limit of the data resource in decimal degrees | 50.75 |
| East | The eastern-most limit of the data resource in decimal degrees | -2 |
| South | The southern-most limit of the data resource in decimal degrees | 50.25 |
| West | The western-most limit of the data resource in decimal degrees | -3.5 |
| Responsible organisations | ||
| Role | The point of contact is person or organisation with responsibility for the creation and maintenance of the metadata for the resource. | pointOfContact |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The originator is the person or organisation who created, collected or produced the resource. | originator |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The custodian is the person or organisation that accepts responsibility for the resource and ensures appropriate care and maintenance. If a dataset has been lodged with a Data Archive Centre for maintenance then this organisation is be entered here. | custodian |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Role | The distributor is the person or organisation that distributes the resource. | distributor |
| Organisation name | Centre for Environment, Fisheries and Aquaculture Science, Lowestoft Laboratory (CEFAS) | |
| Delivery point | Cefas Lowestoft Laboratory, Pakefield Road | |
| Postal code | NR33 0HT | |
| City | Lowestoft | |
| Administrative area | Suffolk | |
| Country | UK | |
| data.manager@cefas.co.uk | ||
| Resource locators | ||
| Locator URL | Web address (URL) that links to the resource | https://data.cefas.co.uk/view/18522 |
| Locator name | Name of the web resource | Cefas Data Portal |
| Dataset constraints | ||
| 20.1 Limitations on Public Access - Access constraints | This states `otherRestrictions` from ISO vocabulary RestrictionCode and is an INSPIRE/GEMINI requirement. | otherRestrictions |
| 20.2 Limitations on Public Access - Other constraints | noLimitations | |
| 21.1 Conditions for Access and Use - Use constraints | This states `otherRestrictions` from ISO vocabulary RestrictionCode and is an INSPIRE/GEMINI requirement. | otherRestrictions |
| 21.2 Conditions for Access and Use - Other constraints | https://www.nationalarchives.gov.uk/doc/open-government-licence/version/3/ | |
| Version info | ||
| Date of publication | The publication date of the resource or if previously unpublished the date that the resource was made publicly available via the MEDIN network. | 2017-05-19 |
| Date of last revision | The most recent date that the resource was revised. | 2024-10-03 |
| Date of creation | The date that the resource was created. | 2017-05-05 |
| Harvest date | The date which this record has been (re)harvested from the provider. | 2026-04-12 |
| Metadata date | The date when the content of this metadata record was last updated. | 2024-10-03 |
| Metadata standard name | The name of the metadata standard used to create this metadata | MEDIN |
| Metadata standard version | The version of the MEDIN Discovery Metadata Standard used to create the metadata record | 3.1.1 |